欢迎进入苏州阿尔法生物实验器材有限公司!
技术文章
首页 > 技术文章 > 琼脂糖预染预制胶说明书

琼脂糖预染预制胶说明书

 更新时间:2023-12-13 点击量:295

*运输及保存

*Shipping and Storage
琼脂糖预染预制胶电泳试剂盒的小黑盒 2~8℃保存和运输,有效期 12 个月。
Ship and store the kit at 2~8℃. It will remain stable for one year.
专适 6×DNA Loading Buffer 2~8℃运输,长期需要-20℃保存,有效期 12 个月。
Ship Optimized 6×DNA loading buffer at 2~8℃,for short-time storage and at -20℃ for long-time storage. It will remain stable for one year.
TAE 速溶颗粒 2~8℃或常温运输,常温保存,有效期 24 个月。
Ship TAE Instant Granule at 2~8℃ or room temperature and store at room temperature. It will remain stable for two years.
专适 Marker 2~8℃运输,长期需要-20℃保存,有效期 12 个月。
Ship Optimized Marker at 2~8℃ and store it at -20°C for long-time storage. It will remain stable for one year.
*自备试剂
*Reagents Required But Not Provided
核酸样品、去离子水
Nucleic acid sample and Deionized water
*使用方法
*Procedure
1.量取约 600ml 的蒸馏水加入烧杯,并放置一个磁性搅拌子于烧杯中。将烧杯置于磁力搅拌器上,慢慢加入 1 袋 TAE 速溶颗粒的全部内容物,搅拌溶液直至
溶解。把烧杯中的溶液倒入 1000ml 的容量瓶中,再加入蒸馏水,定容至 1000ml,即为 1×TAE 溶液。
1. Add one pouch of TAE Instant Granule into the cleaned beaker, dissolved completely with 600 ml distilled water under a magnetic stirrer. Pour the solution into 1L flask. Add distilled water to the
solution until the total volume is 1L. The final solution is 1×TAE buffer.
2.取出一块独立包装的琼脂糖预染预制胶,撕掉表面的塑料膜,反转包装,用两手的食指和中指托住塑料壳边缘,开口向下没入电泳液中,然后用两个大拇指轻
轻按压塑料壳背面中心部分,琼脂糖预染预制胶就会落入电泳液中,此时的预制胶带孔面向上,移动胶块,使孔侧端靠近电泳槽负极。如样品孔内有气泡,应设法除
去。
2. Take out one kit, take off the plastic package, reverse it, support the two edges with index and middle fingers of both hands, immerse it in the buffer with the opening downward and gently press the
central part of the kit with two thumbs. Thus the gel will fall into the buffer with the side of the well upward. Move the gel to make the well end close to negative electrode of the electrophoresis cell. If
bubbles are produced in the sample wells, try to remove them.
3.按 5:1 的比例取适量核酸样品和专适 6×DNA Loading Buffer 混匀,用移液器将专适 Marker 和样品混合液依次缓慢加入被浸没的凝胶加样孔内。
3. Mix Optimized 6×DNA loading buffer and DNA sample at a volume ratio of 1:5. Carefully load prepared Marker and the mixed sample into the wells with pipette successively.
4.接通电源,红色为正极,黑色为负极,切记 DNA 样品由负极往正极泳动(靠近加样孔的一端为负)。
4. Connect the electrophoresis cell to the power source according to the conventions: Red-Anode and Black-Cathode. Turn on the power source. Note that the DNA sample moves from the negative to
the positive (the end near the wells that DNA samples are loaded in is negative).
5.根据指示剂泳动的位置,判断是否终止电泳。
5. Determine whether to stop electrophoresis according to the migration of the tracking dyes.
6.电泳完毕,关闭电源,用凝胶成像仪观察电泳条带及其位置,与 Marker 比较扩增产物的大小。
6. Switch off the power source when the electrophoresis finishes. Visualize the band by using a gel documentation system and compare the size of the amplified product with that of Marker


预制胶取较操作方法




蛋白预制胶取胶方法